Lipid metabolism pathways, particularly steroid hormone biosynthesis and cholesterol metabolism, were highly enriched across all three iHLO development phases, indicating the organoids capacity to process lipids, especially cholesterol
doi: 10.1161/01.RES.86.9.e85
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Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol